Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extraction

Abstract Lipids produced by oleaginous yeasts are considered as sustainable sources for the production of biofuels and oleochemicals. The red yeast Rhodosporidium toruloides can accumulate lipids to over 70% its dry cell mass. To facilitate lipid extraction, a recombinant β-1,3-glucomannanase, MAN5C, has been applied partially breakdown R. wall. In this study, NP11 was engineered secretory expression MAN5C simplify extraction process. Specifically, cassette contained codon-optimized gene integrated into genome Agrobacterium -mediated transformation. strain NP11-MAN5C found with proper secretion active yet no notable compromise in terms growth production. When cultures were extracted ethyl acetate without any pretreatment, 20% total recovered, 4.3-fold higher than that parental NP11. cells heat-treated followed presence culture broth supernatants, up 93% confirming beneficial effects situ. This study provides new strategy engineer more viable down-stream processes. Graphical